Coding
mGL

Part:BBa_K4674000:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-09-30)


mGreenLantern (mGL)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The sequence of mGL is codon optimized for E. Coli expression

Source

mGreenLantern (mGL) is derived from Aequorea victoria

References

mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging Campbell Bc, Nabel Em, Murdock Mh, Lao-Peregrin C, Tsoulfas P, Blackmore Mg, Lee Fs, Liston C, Morishita H, Petsko Ga

(2020). Proceedings of the National Academy of Sciences, , 202000942. doi: 10.1073/pnas.2000942117.